tag:blogger.com,1999:blog-37148773.post8692949318515748557..comments2024-03-27T14:50:47.345-04:00Comments on <center>Sandwalk</center>: Restriction, Modification, and EpigeneticsLarry Moranhttp://www.blogger.com/profile/05756598746605455848noreply@blogger.comBlogger2125tag:blogger.com,1999:blog-37148773.post-3090565475747699952007-09-12T07:33:00.000-04:002007-09-12T07:33:00.000-04:00Hi Peter.Nice clear explanation of restriction/mod...Hi Peter.<BR/>Nice clear explanation of restriction/modofication principles. However, I have a question. If the methylases only recognise hemi-methylated DNA why does NEB EcoRI methylase protect unmethylated plasmid DNA from EcoRI in vitro? Is it simply down to concentration and affinity?Anonymousnoreply@blogger.comtag:blogger.com,1999:blog-37148773.post-62973323104302617932007-02-07T09:37:00.000-05:002007-02-07T09:37:00.000-05:00That was fascinating. And you find the nicest mole...That was fascinating. And you find the nicest molecules to blog about, the inversion symmetry of EcoR1 is beautiful.<br /><br />Keep'em coming, please.Torbjörn Larssonhttps://www.blogger.com/profile/02022193326058378221noreply@blogger.com