tag:blogger.com,1999:blog-37148773.post3648835894287228745..comments2024-03-27T14:50:47.345-04:00Comments on <center>Sandwalk</center>: Ribosomal RNA Genes in EukaryotesLarry Moranhttp://www.blogger.com/profile/05756598746605455848noreply@blogger.comBlogger6125tag:blogger.com,1999:blog-37148773.post-11243428594751211072012-07-05T05:10:18.320-04:002012-07-05T05:10:18.320-04:00Intragenomic variation in ribosomal RNA gene of th...Intragenomic variation in ribosomal RNA gene of the sea urchin Lytechinus variegatus. Molec Biol Rep. Volume 32, Number 1. 61 – 65(2005).N.K.MishraNirmalhttps://www.blogger.com/profile/11197076839025914565noreply@blogger.comtag:blogger.com,1999:blog-37148773.post-68647577261310671372008-01-28T14:15:00.000-05:002008-01-28T14:15:00.000-05:00Looking at the electron micrograph, I'm trying to ...Looking at the electron micrograph, I'm trying to imagine all that transcriptional activity in a three-dimensional ball-and- stick model. I'm then trying to visualise all the various precursors, enzymes and small molecules zipping around while those strands of RNA flail. It's amazing that anything Nature actually works.Anonymousnoreply@blogger.comtag:blogger.com,1999:blog-37148773.post-35758620843504224042008-01-25T10:22:00.000-05:002008-01-25T10:22:00.000-05:00The flatness is just an artifact resulting from th...The flatness is just an artifact resulting from the way the complexes are prepared for the electron microscope. The first step is to dry them out on a flat grid.<BR/><BR/>BTW, in the olden days (late 1960's) we used to call these "Oscar Miller" pictures after the man who first published them.Larry Moranhttps://www.blogger.com/profile/05756598746605455848noreply@blogger.comtag:blogger.com,1999:blog-37148773.post-16285047489744670962008-01-25T02:13:00.000-05:002008-01-25T02:13:00.000-05:00Love the electron micrograph. Classic. But why are...Love the electron micrograph. Classic. But why are the mRNAs splayed out on a 2-D plane? ie Why is the Christmas tree flat?Baymanhttps://www.blogger.com/profile/03436172198266062229noreply@blogger.comtag:blogger.com,1999:blog-37148773.post-61618782822025633882008-01-24T09:58:00.000-05:002008-01-24T09:58:00.000-05:00Thank-you very much for the reference. I'm about t...Thank-you very much for the reference. I'm about to post an article on the human ribosomal RNA gene arrays and that reference is crucial.Larry Moranhttps://www.blogger.com/profile/05756598746605455848noreply@blogger.comtag:blogger.com,1999:blog-37148773.post-10952585412426268562008-01-24T09:17:00.000-05:002008-01-24T09:17:00.000-05:00High-fidelity gene arrays like these clusters pres...High-fidelity gene arrays like these clusters present special challenges for genome sequencing efforts, since shotgun assembly of such highly repetitive sequences is difficult or impossible. The human genome project for instance doesn't have any information on the larger rDNA arrays, and is greatly abbreviated (read: incorrect) with respect to the 5S arrays.<BR/><BR/>This sequence assembly difficulty is unfortunate because lack of genome project representation tends to hide the really tremendous human genomic variation at these loci.<BR/><BR/>See for example:<BR/>Stults DM, Killen MW, Pierce HH, Pierce AJ.<BR/>Genomic architecture and inheritance of human ribosomal RNA gene clusters.<BR/>Genome Res. 2008 Jan;18(1):13-8. Epub 2007 Nov 19.Anonymousnoreply@blogger.com