tag:blogger.com,1999:blog-37148773.post4894915671225377968..comments2024-03-27T14:50:47.345-04:00Comments on <center>Sandwalk</center>: Regulating Enzyme Activity by Covalent ModificationLarry Moranhttp://www.blogger.com/profile/05756598746605455848noreply@blogger.comBlogger7125tag:blogger.com,1999:blog-37148773.post-70335219348799032812007-11-29T13:45:00.000-05:002007-11-29T13:45:00.000-05:00The codons are UGU and UGC—the Cys codons. Those a...The codons are UGU and UGC—the Cys codons. Those are the ones recognized by phosphoseryl-tRNA because the phosphoserine is converted to cysteine while it's still attached to tRNA but before it is incorporated into protein.<BR/>********************************<BR/><BR/>The codons though are still for Cys not phosphoserine. Codons are defined by what amino acid they code for in translation not based on what amino acid gets attached to the corresponding tRNA.PonderingFoolhttps://www.blogger.com/profile/10767758746935185528noreply@blogger.comtag:blogger.com,1999:blog-37148773.post-39501733233044756132007-11-29T10:25:00.000-05:002007-11-29T10:25:00.000-05:00I have found what I think is a complete list of ph...I have found what I think is a complete list of phosphorylated amino acids and incorporated that information into the post.<BR/><BR/>Thanks to those who added examples.<BR/><BR/>I checked with a colleague before posting and he couldn't think of any examples other than S, T, and Y. We agreed that my readers would soon point out the errors of our ways if we were wrong.<BR/><BR/>Isn't blogging wonderful? It's a really good way to learn things.Larry Moranhttps://www.blogger.com/profile/05756598746605455848noreply@blogger.comtag:blogger.com,1999:blog-37148773.post-19583577302779804602007-11-29T10:11:00.000-05:002007-11-29T10:11:00.000-05:00ponderingfool says, ... even though phosphoserine ...ponderingfool says,<BR/><BR/><I> ... even though phosphoserine is found in proteins and an aminoacyl-tRNA synthetasethat attaches the amino acid to tRNAs is found in nature, no known codon for phosphoserine exists.</I><BR/><BR/>The codons are UGU and UGC—the Cys codons. Those are the ones recognized by phosphoseryl-tRNA because the phosphoserine is converted to cysteine while it's still attached to tRNA but before it is incorporated into protein.Larry Moranhttps://www.blogger.com/profile/05756598746605455848noreply@blogger.comtag:blogger.com,1999:blog-37148773.post-37962024913402789172007-11-29T07:34:00.000-05:002007-11-29T07:34:00.000-05:00Since we're listing phosphorylated amino acid side...Since we're listing phosphorylated amino acid side chains, let's not forget aspartate.Anonymousnoreply@blogger.comtag:blogger.com,1999:blog-37148773.post-69833488046854576382007-11-28T20:52:00.000-05:002007-11-28T20:52:00.000-05:00Just to throw some extra trivia in there, even tho...Just to throw some extra trivia in there, even though phosphoserine is found in proteins and <A HREF="http://www.ncbi.nlm.nih.gov/sites/entrez?Db=pubmed&Cmd=ShowDetailView&TermToSearch=15790858&ordinalpos=4&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_RVDocSum" REL="nofollow">an aminoacyl-tRNA synthetase</A>that attaches the amino acid to tRNAs is found in nature, no known codon for phosphoserine exists. Biology is far more interesting than anything that is designed, IMHO.PonderingFoolhttps://www.blogger.com/profile/10767758746935185528noreply@blogger.comtag:blogger.com,1999:blog-37148773.post-24589211825498522032007-11-28T19:57:00.000-05:002007-11-28T19:57:00.000-05:00For the sake of completeness, amino acids other th...For the sake of completeness, amino acids other than S,T,Y can be and apparently are reversibly phosphorylated, notably histidine.Anonymousnoreply@blogger.comtag:blogger.com,1999:blog-37148773.post-52757997928669052182007-11-28T15:27:00.000-05:002007-11-28T15:27:00.000-05:00Wow...can't believe I missed this one, what with m...Wow...can't believe I missed this one, what with my Ph.D. in...protein phosphorylation!Mikehttps://www.blogger.com/profile/09297263039516044615noreply@blogger.com