tag:blogger.com,1999:blog-37148773.post1423027367993888572..comments2024-03-27T14:50:47.345-04:00Comments on <center>Sandwalk</center>: Monday's Molecule #153Larry Moranhttp://www.blogger.com/profile/05756598746605455848noreply@blogger.comBlogger6125tag:blogger.com,1999:blog-37148773.post-13797086383560765082011-12-15T15:17:34.773-05:002011-12-15T15:17:34.773-05:00The structure is a beta-glucosylated hydroxymethyl...The structure is a beta-glucosylated hydroxymethylcytosine. Bacteriophage T4 beta-glucosyltransferase tacks on a glucosyl residue on the -OH group of the hydroxymethylC. When found in DNA, this modification of 5-OH-methylC confers resistance of DNA specific nucleasesSalhttps://www.blogger.com/profile/05479312013429407926noreply@blogger.comtag:blogger.com,1999:blog-37148773.post-85515783267522214772011-12-15T09:14:49.923-05:002011-12-15T09:14:49.923-05:00glc-HMC. It makes T4 phage resistant to E. coli r...glc-HMC. It makes T4 phage resistant to E. coli restriction enzymes, though this has been overcome by gmrSD, which is in turn overcome by IPI*.Matt Gnoreply@blogger.comtag:blogger.com,1999:blog-37148773.post-12827013587187315412011-12-14T22:18:15.597-05:002011-12-14T22:18:15.597-05:00Molecule #153 is 5-gmC, or β-glycosyl-5-hydroxymet...Molecule #153 is 5-gmC, or β-glycosyl-5-hydroxymethylcytosine, or β-D-glucopyranosyl-6-amino-5-(hydroxymethyl)-1H-pyrimidin-2-one. <br />It is also the glucoside of 5-hydroxymethylcytosine (5-hmC), found in mammalian cells, especially in the central nervous system, and whose presence in the genome has been associated with gene activation and epigenetics.Raul A. Félix de Sousanoreply@blogger.comtag:blogger.com,1999:blog-37148773.post-36729963549377340162011-12-14T16:06:13.517-05:002011-12-14T16:06:13.517-05:00I believe the molecule is beta-glucosyl-5-hydroxym...I believe the molecule is beta-glucosyl-5-hydroxymethylcytosine. It is found in Enterobacteria phage T4, also known as bacteriophage T4.<br /><br />The enzyme DNA beta-glucosyltransferase catalyzes the transfer of glucose from UDP-glucose to 5-hydroxymethylcytosine (present in phage DNA in place of cytosine) in the double-stranded DNA of the T4 phage. This prevents restriction endonucleases found in its host (E. coli) from recognizing its DNA (at a restriction site containing the glucosylated base) and cleaving it.<br /><br />-Jason OakleyJason Oakleyhttps://www.blogger.com/profile/14414529190965164341noreply@blogger.comtag:blogger.com,1999:blog-37148773.post-46184278663482664802011-12-14T15:46:47.309-05:002011-12-14T15:46:47.309-05:00I think the molecule is (beta)-L-glucosyl-5-hydrox...I think the molecule is (beta)-L-glucosyl-5-hydroxymethylcytosine.<br /><br />The enzyme is DNA beta-glucosyltransferase. Functionally, it transfers the glucose from uridine diphosphoglucose to 5-hydroxymethylcytosine in T4 phage DNA.<br /><br />It's found in the DNA of T4 bacteriophage, where it protects the phage DNA from phage and host derived nucleases (restriction endonucleases)Christopher Yauhttps://www.blogger.com/profile/00359246621463321318noreply@blogger.comtag:blogger.com,1999:blog-37148773.post-79330392396778214702011-12-14T14:24:17.743-05:002011-12-14T14:24:17.743-05:00Larry-
The molecule is beta-glucosyl-5-hydroxymet...Larry-<br /><br />The molecule is beta-glucosyl-5-hydroxymethylcytosine.<br /><br />The "species" it is found in is T4 bacteriophage.<br /><br />It is the product of a beta-glucosyltransferase utilizing UDP-glucose as a substrate to transfer glucose to hydroxymethylcytosine (formed from cytosine) in the T4 DNA to enable it to evade host restriction nuclease enzyme attack.<br /><br />Best wishes,<br />Bill ChaneyBill Chaneynoreply@blogger.com